TRAF interacting protein

Protein-coding gene in the species Homo sapiens
TRAIP
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

4ZTD

Identifiers
AliasesTRAIP, RNF206, TRIP, SCKL9, TRAF interacting protein
External IDsOMIM: 605958; MGI: 1096377; HomoloGene: 31343; GeneCards: TRAIP; OMA:TRAIP - orthologs
Gene location (Human)
Chromosome 3 (human)
Chr.Chromosome 3 (human)[1]
Chromosome 3 (human)
Genomic location for TRAIP
Genomic location for TRAIP
Band3p21.31Start49,828,601 bp[1]
End49,856,574 bp[1]
Gene location (Mouse)
Chromosome 9 (mouse)
Chr.Chromosome 9 (mouse)[2]
Chromosome 9 (mouse)
Genomic location for TRAIP
Genomic location for TRAIP
Band9 F1|9 59.07 cMStart107,827,335 bp[2]
End107,849,469 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • testicle

  • gonad

  • ventricular zone

  • ganglionic eminence

  • mucosa of transverse colon

  • left testis

  • right testis

  • stromal cell of endometrium

  • rectum

  • nucleus accumbens
Top expressed in
  • tail of embryo

  • gastrula

  • genital tubercle

  • fetal liver hematopoietic progenitor cell

  • morula

  • morula

  • otic placode

  • zygote

  • yolk sac

  • embryo
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • ubiquitin-protein transferase activity
  • protein binding
  • metal ion binding
  • ubiquitin protein ligase activity
  • transferase activity
  • zinc ion binding
Cellular component
  • cytoplasm
  • perinuclear region of cytoplasm
  • nucleolus
  • nucleus
Biological process
  • cell population proliferation
  • protein ubiquitination
  • negative regulation of interferon-beta production
  • signal transduction
  • negative regulation of tumor necrosis factor-mediated signaling pathway
  • apoptotic process
  • negative regulation of NF-kappaB transcription factor activity
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

10293

22036

Ensembl

ENSG00000183763

ENSMUSG00000032586

UniProt

Q9BWF2

Q8VIG6

RefSeq (mRNA)

NM_005879

NM_011634

RefSeq (protein)

NP_005870

NP_035764

Location (UCSC)Chr 3: 49.83 – 49.86 MbChr 9: 107.83 – 107.85 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
The AlphaFold predicted structure of the TRAIP protein Q9BWF2. The highlighted region has been found to bind to the PCNA PIP box. This image was generated using ChimeraX version 1.7.1.
Detailed view of residue sequence 447-469 of the TRAIP protein in its binding conformation with the PCNA PIP box.[5] This This image was generated using ChimeraX version 1.7.1.

TRAF-interacting protein is a protein that in humans is encoded by the TRAIP gene.[6][7]

This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.[7]

Interactions

TRAF interacting protein has been shown to interact with FLII,[8] TRAF1[6] and TRAF2.[6]

Role in mitotic DNA synthesis

Mitotic DNA synthesis (MiDAS) is thought to be a DNA repair mechanism to salvage DNA that has not finished replication during S phase, which may be due to DNA replication stress (RS).[9] Intrinsic sources of RS include transcription-replication conflicts and “difficult-to-replicate’’ regions.[9] Extrinsic RS includes exposure to genotoxic agents, depletion of dNTPs, and premature S phase activity which can occur in precancerous cells after oncogene activation.[9] Some MiDAS pathways require the TRAIP protein to disassemble the replication complex at the stalled replication fork in cases where RS causes the fork to stall during replication.[9]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000183763 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000032586 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Hoffmann S, Smedegaard S, Nakamura K, Mortuza GB, Räschle M, Ibañez de Opakua A, et al. (January 2016). "TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress". The Journal of Cell Biology. 212 (1): 63–75. doi:10.1083/jcb.201506071. PMC 4700480. PMID 26711499.
  6. ^ a b c Lee SY, Lee SY, Choi Y (April 1997). "TRAF-interacting protein (TRIP): a novel component of the tumor necrosis factor receptor (TNFR)- and CD30-TRAF signaling complexes that inhibits TRAF2-mediated NF-kappaB activation". The Journal of Experimental Medicine. 185 (7): 1275–1285. doi:10.1084/jem.185.7.1275. PMC 2196258. PMID 9104814.
  7. ^ a b "Entrez Gene: TRAIP TRAF interacting protein".
  8. ^ Wilson SA, Brown EC, Kingsman AJ, Kingsman SM (August 1998). "TRIP: a novel double stranded RNA binding protein which interacts with the leucine rich repeat of flightless I". Nucleic Acids Research. 26 (15): 3460–3467. doi:10.1093/nar/26.15.3460. PMC 147727. PMID 9671805.
  9. ^ a b c d Bhowmick R, Hickson ID, Liu Y (October 2023). "Completing genome replication outside of S phase". Molecular Cell. 83 (20): 3596–3607. doi:10.1016/j.molcel.2023.08.023. PMID 37716351.

Further reading

  • Beckly JB, Hancock L, Geremia A, Cummings JR, Morris A, Cooney R, et al. (April 2008). "Two-stage candidate gene study of chromosome 3p demonstrates an association between nonsynonymous variants in the MST1R gene and Crohn's disease". Inflammatory Bowel Diseases. 14 (4): 500–507. doi:10.1002/ibd.20365. PMID 18200509. S2CID 37697301.
  • Regamey A, Hohl D, Liu JW, Roger T, Kogerman P, Toftgard R, et al. (December 2003). "The tumor suppressor CYLD interacts with TRIP and regulates negatively nuclear factor kappaB activation by tumor necrosis factor". The Journal of Experimental Medicine. 198 (12): 1959–1964. doi:10.1084/jem.20031187. PMC 2194148. PMID 14676304.
  • Wilson SA, Brown EC, Kingsman AJ, Kingsman SM (August 1998). "TRIP: a novel double stranded RNA binding protein which interacts with the leucine rich repeat of flightless I". Nucleic Acids Research. 26 (15): 3460–3467. doi:10.1093/nar/26.15.3460. PMC 147727. PMID 9671805.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–174. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.

External links

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