MCM3AP

Protein-coding gene in the species Homo sapiens
MCM3AP
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

4DHX

Identifiers
AliasesMCM3AP, GANP, MAP80, SAC3, minichromosome maintenance complex component 3 associated protein, PNRIID
External IDsOMIM: 603294; MGI: 1930089; HomoloGene: 2902; GeneCards: MCM3AP; OMA:MCM3AP - orthologs
Gene location (Human)
Chromosome 21 (human)
Chr.Chromosome 21 (human)[1]
Chromosome 21 (human)
Genomic location for MCM3AP
Genomic location for MCM3AP
Band21q22.3Start46,235,133 bp[1]
End46,286,297 bp[1]
Gene location (Mouse)
Chromosome 10 (mouse)
Chr.Chromosome 10 (mouse)[2]
Chromosome 10 (mouse)
Genomic location for MCM3AP
Genomic location for MCM3AP
Band10|10 C1Start76,304,761 bp[2]
End76,351,691 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • tendon of biceps brachii

  • tibial nerve

  • right uterine tube

  • gastrocnemius muscle

  • body of uterus

  • gastric mucosa

  • right ovary

  • right lobe of thyroid gland

  • cerebellar hemisphere

  • right hemisphere of cerebellum
Top expressed in
  • saccule

  • otic vesicle

  • zygote

  • primary oocyte

  • otic placode

  • secondary oocyte

  • Rostral migratory stream

  • granulocyte

  • renal corpuscle

  • ventricular zone
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • transferase activity
  • DNA binding
  • acyltransferase activity
  • nucleic acid binding
  • chromatin binding
  • histone acetyltransferase activity
  • protein binding
  • H3 histone acetyltransferase activity
  • histone binding
Cellular component
  • cytosol
  • nuclear pore
  • nuclear membrane
  • nuclear envelope
  • nucleus
  • nucleoplasm
  • cytoplasm
  • transcription export complex 2
  • nuclear pore nuclear basket
  • chromosome
Biological process
  • protein transport
  • mRNA transport
  • protein import into nucleus
  • immune system process
  • transport
  • mRNA export from nucleus
  • poly(A)+ mRNA export from nucleus
  • somatic hypermutation of immunoglobulin genes
  • histone acetylation
  • nucleosome organization
  • histone H3 acetylation
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

8888

54387

Ensembl

ENSG00000160294

ENSMUSG00000001150

UniProt

O60318

Q9WUU9

RefSeq (mRNA)

NM_003906

NM_019434

RefSeq (protein)

NP_003897

NP_062307

Location (UCSC)Chr 21: 46.24 – 46.29 MbChr 10: 76.3 – 76.35 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene.[5][6]

Function

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is an MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization.[6]

Interactions

MCM3AP has been shown to interact with:

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000160294 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000001150 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b Takei Y, Tsujimoto G (September 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
  6. ^ a b "Entrez Gene: MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein".
  7. ^ Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
  8. ^ Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID 10733502.
  9. ^ Zhou Y, Li L, Liu Q, Xing G, Kuai X, Sun J, Yin X, Wang J, Zhang L, He F (May 2008). "E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3". Cell. Signal. 20 (5): 942–8. doi:10.1016/j.cellsig.2008.01.010. PMID 18276110.

Further reading

  • Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
  • Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID 10733502.
  • Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N (2000). "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication". Gene. 255 (2): 219–27. doi:10.1016/S0378-1119(00)00336-X. PMID 11024281.
  • Takei Y, Swietlik M, Tanoue A, Tsujimoto G, Kouzarides T, Laskey R (2001). "MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3". EMBO Rep. 2 (2): 119–23. doi:10.1093/embo-reports/kve026. PMC 1083822. PMID 11258703.
  • Kuwahara K, Tomiyasu S, Fujimura S, Nomura K, Xing Y, Nishiyama N, Ogawa M, Imajoh-Ohmi S, Izuta S, Sakaguchi N (2001). "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions". Proc. Natl. Acad. Sci. U.S.A. 98 (18): 10279–83. Bibcode:2001PNAS...9810279K. doi:10.1073/pnas.181335698. PMC 56952. PMID 11526238.
  • EL-Gazzar MA, Maeda K, Nomiyama H, Nakao M, Kuwahara K, Sakaguchi N (2001). "PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP". J. Biol. Chem. 276 (51): 48000–8. doi:10.1074/jbc.M106696200. PMID 11641399.
  • Kono Y, Maeda K, Kuwahara K, Yamamoto H, Miyamoto E, Yonezawa K, Takagi K, Sakaguchi N (2002). "MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR". Genes Cells. 7 (8): 821–34. doi:10.1046/j.1365-2443.2002.00562.x. PMID 12167160. S2CID 21946802.
  • Takei Y, Assenberg M, Tsujimoto G, Laskey R (2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
  • Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
  • Sakaguchi N, Fujimura S, Kuwahara K (2002). "Involvement of GANP in B cell activation in T cell-dependent antigen response". Dev. Immunol. 9 (3): 169–72. doi:10.1080/1044667031000137647. PMC 2276099. PMID 12885157.
  • Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
  • Hu YH, Warnatz HJ, Vanhecke D, Wagner F, Fiebitz A, Thamm S, Kahlem P, Lehrach H, Yaspo ML, Janitz M (2006). "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins". BMC Genomics. 7: 155. doi:10.1186/1471-2164-7-155. PMC 1526728. PMID 16780588.

External links

  • PDBe-KB provides an overview of all the structure information available in the PDB for Human Germinal-center associated nuclear protein (MCM3AP)


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