5-carboxymethyl-2-hydroxymuconate Delta-isomerase

In enzymology, a 5-carboxymethyl-2-hydroxymuconate Delta-isomerase (EC 5.3.3.10) is an enzyme that catalyzes the chemical reaction

5-carboxymethyl-2-hydroxymuconate ${\displaystyle \rightleftharpoons }$ 5-carboxy-2-oxohept-3-enedioate

Hence, this enzyme has one substrate, 5-carboxymethyl-2-hydroxymuconate, and one product, 5-carboxy-2-oxohept-3-enedioate.

This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases transposing C=C bonds. The systematic name of this enzyme class is 5-carboxymethyl-2-hydroxymuconate Delta2,Delta4-2-oxo,Delta3-isomerase. This enzyme participates in tyrosine metabolism and benzoate degradation via hydroxylation.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1GTT, 1I7O, 1WZO, and 2DFU.

References

• Garrido-Peritierra A, Cooper RA (July 1981). "Identification and purification of distinct isomerase and decarboxylase enzymes involved in the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli". European Journal of Biochemistry. 117 (3): 581–4. doi:10.1111/j.1432-1033.1981.tb06377.x. PMID 7026235.